cd73 (nt5e) antibody, anti-human, readye_lease  (Miltenyi Biotec)

Journal: bioRxiv

Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells

doi: 10.64898/2026.06.25.733625

Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay

Journal: bioRxiv

Article Title: Donor-matched iPSC model reveals context-dependent T2D genetic signals in fibro-adipogenic progenitors

doi: 10.64898/2026.02.04.702388

Figure Lengend Snippet: A – A heatmap comparing reference genotyping data from the FUSION study to low-pass genotyping data generated as part of QC metrics for this study. The fraction match indicates the fraction of reads that aligned from the low pass WGS data to the reference SNP Chip. B – Histogram of FAP marker gene expression based on FACS assessment of 30 different lines. The X-axis indicates the percentage of cells per line expressing NT5E. The average across all cell lines was 74% of cells expressing the FAP marker gene. C – Sankey plot representing the insulin-stimulated glucose uptake assay. D – Comparison of normalized luminescence from all 30 donors (with replicates) in basal, basal with a small dose of insulin, high-insulin, and high-insulin with a small additional dose of insulin. Pink lines indicate the average luminescence value for that environmental condition. A paired Student’s t-test was used to assess statistical significance. E – An interval plot comparing donor metadata to luminescence levels in basal conditions. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM. F – An interval plot comparing donor metadata to luminescence levels in high-insulin conditions. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM. G – An interval plot comparing donor metadata to luminescence levels in basal conditions with an additional small dose of insulin. Data represented as mean ± SEM. H – An interval plot comparing donor metadata to luminescence levels in high-insulin conditions with an additional small dose of insulin. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM.

Article Snippet: Cells are then centrifuged down, media is aspirated, and the positive sample is stained with antibodies for Tra-1-60 (iPSC marker) (Miltenyi Biotec Cat# 130-122-921, RRID:AB_2801969) and NT5E (FAP marker) (Miltenyi Biotec Cat# 130-111-913, RRID:AB_2784275) for 10 minutes.

Techniques: Generated, Marker, Gene Expression, Expressing, Comparison